Contribution of FcɛRI-associated vesicles to mast cell–macrophage communication following Francisella tularensis infection:

Innate Immunity

Annette RRodriguez¹ 

关键词: FcɛRI;    Francisella;    macrophages;    mast cells;    trogocytosis;   

DOI  :  10.1177/1753425916663639

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Understanding innate immune intercellular communication following microbial infection remains a key biological issue. Using live cell imaging, we demonstrate that mast cells actively extend cellular projections to sample the macrophage periphery during Francisella tularensis LVS infection. Mast cell MHCIIhi expression was elevated from less than 1% to 13% during LVS infection. Direct contact during co-culture with macrophages further increased mast cell MHCIIhi expression to approximately 87%. Confocal analyses of the cellular perimeter revealed mast cell caspase-1 was localized in close proximity with FcɛRI in uninfected mast cells, and repositioned to clustered regions upon LVS infection. Importantly, mast cell FcɛRI-encompassed vesicles are transferred to macrophages by trogocytosis, and macrophage caspase-1 expression is further up-regulated upon direct contact with mast cells. Our study reveals direct cellular interactions between innate cells that may impact the function of caspase-1, a known sensor...

【 授权许可】

CC BY