Introgression of Chromosome 3Ns from Psathyrostachys huashanica into Wheat Specifying Resistance to Stripe Rust

PLoS One

Haiqin Zhang¹  George Fedak¹  Wenguang Cao¹  Yi Wang²  Houyang Kang²  Yonghong Zhou²  Xing Fan²  Youliang Zheng²  Lili Xu²  Lina Sha² 

[1]Eastern Cereal and Oilseed Research Centre, Department of Agriculture and Agriculture-Food Canada, Ottawa, Ontario, Canada

[2]Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China

关键词: Wheat;    Chromosomal translocations;    Introgression;    C banding;    Cereal crops;    Chromatin;    Chromosome pairs;    Invasive species;   

DOI  :  10.1371/journal.pone.0021802

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Wheat stripe rust is a destructive disease in the cool and humid wheat-growing areas of the world. Finding diverse sources of stripe rust resistance is critical for increasing genetic diversity of resistance for wheat breeding programs. Stripe rust resistance was identified in the alien species Psathyrostachys huashanica, and a wheat- P. huashanica amphiploid line (PHW-SA) with stripe rust resistance was reported previously. In this study, a P. huashanica 3Ns monosomic addition line (PW11) with superior resistance to stripe rust was developed, which was derived from the cross between PHW-SA and wheat J-11. We evaluated the alien introgressions PW11-2, PW11-5 and PW11-8 which were derived from line PW11 for reaction to new Pst race CYR32, and used molecular and cytogenetic tools to characterize these lines. The introgressions were remarkably resistant to CYR32, suggesting that the resistance to stripe rust of the introgressions thus was controlled by gene(s) located on P. huashanica chromosome 3Ns. All derived lines were cytologically stable in term of meiotic chromosome behavior. Two 3Ns chromosomes of P. huashanica were detected in the disomic addition line PW11-2. Chromosomes 1B of substitution line PW11-5 had been replaced by a pair of P. huashanica 3Ns chromosomes. In PW11-8, a small terminal segment from P. huashanica chromosome arm 3NsS was translocated to the terminal region of wheat chromosomes 3BL. Thus, this translocated chromosome is designated T3BL-3NsS. These conclusions were further confirmed by SSR analyses. Two 3Ns-specific markers Xgwm181 and Xgwm161 will be useful to rapidly identify and trace the translocated fragments. These introgressions, which had significant characteristics of resistance to stripe rust, could be utilized as novel germplasms for wheat breeding.

【 授权许可】

CC BY