Analysis of Host Gene Expression Profile in HIV-1 and HIV-2 Infected T-Cells

PLoS One

Krishnakumar Devadas¹  Viswanath Ragupathy¹  Indira Hewlett¹  Santanu Biswas¹  Sherwin Lee¹  Mohan Haleyurgirisetty¹  Owen Wood¹ 

[1] Laboratory of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, 10903 New Hampshire Ave, Silver Spring, Maryland, 20993–0002, United States of America

关键词: HIV-1;    Gene expression;    HIV-2;    Gene regulation;    Viral gene expression;    Virus effects on host gene expression;    Microarrays;    Gene ontologies;   

DOI  :  10.1371/journal.pone.0147421

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

HIV replication is closely regulated by a complex pathway of host factors, many of them being determinants of cell tropism and host susceptibility to HIV infection. These host factors are known to exert a positive or negative influence on the replication of the two major types of HIV, HIV-1 and HIV-2, thereby modulating virus infectivity, host response to infection and ultimately disease progression profiles characteristic of these two types. Understanding the differential regulation of host cellular factors in response to HIV-1 and HIV-2 infections will help us to understand the apparent differences in rates of disease progression and pathogenesis. This knowledge would aid in the discovery of new biomarkers that may serve as novel targets for therapy and diagnosis. The objective of this study was to determine the differential expression of host genes in response to HIV-1/HIV-2 infection. To achieve this, we analyzed the effects of HIV-1 (MN) and HIV-2 (ROD) infection on the expression of host factors in PBMC at the RNA level using the Agilent Whole Human Genome Oligo Microarray. Differentially expressed genes were identified and their biological functions determined. Host gene expression profiles were significantly changed. Gene expression profiling analysis identified a subset of differentially expressed genes in HIV-1 and HIV-2 infected cells. Genes involved in cellular metabolism, apoptosis, immune cell proliferation and activation, cytokines, chemokines, and transcription factors were differentially expressed in HIV-1 infected cells. Relatively few genes were differentially expressed in cells infected with HIV-2.

【 授权许可】

CC BY