Inducible factors with antimicrobial activity after immune challenge in the haemolymph of Red Palm Weevil (Insecta):

Innate Immunity

MaristellaMastore¹ 

关键词: Antimicrobial peptides;    innate immunity;    insects;    coleopteran;    alternative drugs;   

DOI  :  10.1177/1753425914542446

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Insects are capable of innate immune responses elicited after microbial infection. In this process, the receptor-mediated recognition of foreign bodies and the subsequent activation of immunocompetent cells lead to the synthesis ex novo of a peptide pool with antimicrobial activity. We investigated the inducible immune response of a coleopteran, Rhynchophorus ferrugineus, challenged with both Gram-negative and Gram-positive bacteria. After immunization, we evaluated the presence of antimicrobial peptides using either biochemical analyses or microbiological techniques. The antimicrobial properties of the newly synthesized protein pool, detectable in haemolymph fractions of low molecular mass, showed strong antibacterial activity against various bacterial strains (Escherichia coli, Pseudomonas sp. OX1, Bacillus subtilis and Micrococcus luteus). In addition to the preliminary study of the mechanism of action of the pool of antimicrobial peptides, we also investigated its effects on bacterial cell walls by me...

【 授权许可】

CC BY