Изучение состава фенольных соединений володушки козелецелистной (Bupleurum scorzonerifolium Willd.), произрастающей в Прибайкалье, методом ВЭЖХ

Acta Biomedica Scientifica

С. А. Петухова¹  В. М. Мирович¹  Л. В. Дударева² 

[1] ФГБОУ ВО «Иркутский государственный медицинский университет» Минздрава России;ФГБУН Сибирский институт физиологии и биохимии растений СО РАН

关键词: ВЭЖХ;    фенольные соединения;    флавоноиды;    дубильные вещества;    фенолкарбоновые кислоты;    володушка козелецелистная;   

DOI  :  10.12737/article_59f035fe65fc57.52277900

学科分类：自然科学（综合）

来源: Scientific entre for Family Health and Human Reproduction Problems

【 摘 要 】

Phenolic compounds of plants have a wide spectrum of pharmacological action. A number of effective herbal medicines and biologically active additives have been developed on the basis of raw materials and natural polyphenols. The sources of phenolic compounds are plants of the genus Bupleurum L. In Russia Bupleurum scorzonerifolium used in folk medicine. Infusions and decoctions were taken for diseases of the liver, gall bladder, topically as an anti-inflammatory agent. The composition of phenolic compounds in the above-ground organs of the B. scorzonerifolium studied by HPLC on the device "GILSTON". Samples of raw materials (above-ground organs) were collected in the southern areas of the Irkutsk region. For the analysis of the herb B. scorzonerifolium 70% extract ethyl alcohol was used. Separation was performed on substances Kromasil C18 column (4,6 x 250 mm, 5 micron). Detection was carried out using a UV detector "GILSTON" UV/VIS model 151, at a wavelength of 254 nm. In comparison with standard samples we identified 8 flavonoids (prevailing - izoquercetin, hyperoside, izoramnetin-3-glucoside), 5 aromatic acids (predominantly acid - cinnamic, gallic, chicory). The study established the content of epicatechin, catechin, epigallocatechingallate, coumarin. For the first time the content of acids - coffee, chlorogenic, gallic, chicory, cinnamon; flavonoids - vitsenin, lyuteolin-7-glucoside, isoramnetin-3-glucoside, apigenin; epicatechin, catechin, epigallocatechingallate; coumarin -was established in B. scorzonerifolium.

【 授权许可】

CC BY