Altered innate immune response in farmers and smokers:

Innate Immunity

KarinSahlander¹ 

关键词: Toll-like receptors;    cytokines;    helper T-cells;    inflammation;    organic dust;    lipopolysaccharide;    peptidoglycan;    farmer;    cigarette smoker;   

DOI  :  10.1177/1753425909106317

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Pig farmers and cigarette smokers are continuously exposed to pathogen-associated molecular patterns (PAMPs) have an increased prevalence of respiratory disorders, such as chronic bronchitis and chronic obstructive pulmonary decease (COPD). We hypothesized that markers of innate immunity, T-helper (Th) cell cytokine profile and acute responses to pro-inflammatory stimuli differ between smokers and farmers, who are exposed to organic material on a daily basis and healthy non-exposed subjects. Eleven non-smoking pig farmers, 12 non-farming smokers and 12 controls underwent bronchial lipopolysaccharide (LPS) challenge and exposure in a pig barn during 3 h on separate days. Toll-like receptor 2 (TLR2), TLR4 and CD14 on blood monocytes and neutrophils and intracellular cytokine profile of Th cells were assessed before and 7 h after exposures. The same outcomes were analysed on peripheral blood and purified neutrophils from farmers and controls after stimulation ex vivo with dust from a pig barn and LPS. Circul...

【 授权许可】

CC BY