Первые результаты изучения «клещевых» инфекций на доклинической стадии инфицирования человека

Acta Biomedica Scientifica

Ж. .. Батаа¹  Н. А. Ляпунова²  И. С. Манзарова²  М. А. Хаснатинов²  И. С. Соловаров²  И. В. Петрова²  Г. А. Данчинова²  А. В. Ляпунов² 

[1] Институт традиционной медицины, Монгольский национальный университет медицинских наук;ФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»

关键词: клещевые инфекции;    клещевой энцефалит;    иксодовый клещевой боррелиоз;    моноцитарный эрлихиоз человека;    гранулоцитарный анаплазмоз человека;    образцы крови;    цитокины;   

DOI  :  10.29413/ABS.2018-3.1.6

学科分类：自然科学（综合）

来源: Scientific entre for Family Health and Human Reproduction Problems

【 摘 要 】

Background. Early detection of tick-borne pathogens in blood samples of infected people prevents the disease before clinical manifestations using antibiotic and immunoglobulin treatment. As far as tick-borne diseases are associated with significant activation of innate immune response, the changes in the cytokine profile soon after a tick bite could be used as an indicator of early tick-borne infection. Aims. The goal of this study was to characterize the production of cytokines during first 2-4 days after a tick bite in asymptomatic people infected with tick-borne pathogens. Materials and methods. The infection of tick-borne encephalitis virus, B. burgdorferi sensu lato, A. phagocytophilum and E. chaffeensis / E. muris was detected in blood of bitten humans using appropriate commercial real time RT-PCR and ELISA assays. Concentration of 14 cytokines in plasma of 89 infected and 45 non-infected people was established using quantitative ELISA kits. Results. Between 2 ndand 4 thdays after tick bite, the prevalence and concentration of certain cytokines in blood of infected people significantly differed from those of the control group. Bacterial pathogens exhibited very similar patterns of cytokine profiles with induction of IL-1a, IL-8 и IFN-a and suppression of SOD, IL-1Ra и IL-17A. For virus infection the increased concentrations of IL-1 a, IL-8, IL-10, IL-17A, IFN-y and SOD were detected. The only suppressed cytokine was IL-1Ra receptor antagonist. Conclusions. The results suggest that evaluation of innate immune response between 2 ndand 4 thdays after the tick bite could be a useful tool for evaluation of the risk of tick-borne disease for the humans bitten by ticks.

【 授权许可】

CC BY