Distinct TLR-mediated cytokine production and immunoglobulin secretion in human newborn naïve B cells:

Innate Immunity

Matthew APettengill¹ 

关键词: B lymphocyte;    TLR;    cytokine;    neonatal;    class-switch recombination;   

DOI  :  10.1177/1753425916651985

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Neonatal innate immunity is distinct from that of adults, which may contribute to increased susceptibility to infection and limit vaccine responses. B cells play critical roles in protection from infection and detect PAMPs via TLRs, that, when co-activated with CD40, can drive B-cell proliferation and Ab production. We characterized the expression of TLRs in circulating B cells from newborns and adults, and evaluated TLR- and CD40-mediated naïve B-cell class-switch recombination (CSR) and cytokine production. Gene expression levels of most TLRs was similar between newborn and adult B cells, except that newborn naïve B cells expressed more TLR9 than adult naïve B cells. Neonatal naïve B cells demonstrated impaired TLR2- and TLR7- but enhanced TLR9-mediated cytokine production. Significantly fewer newborn naïve B cells underwent CSR to produce IgG, an impairment also noted with IL-21 stimulation. Additionally, co-stimulation via CD40 and TLRs induced greater cytokine production in adult B cells. Thus, while...

【 授权许可】

CC BY