Adipocyte-macrophage interaction may mediate LPS-induced low-grade inflammation: Potential link with metabolic complications:

Innate Immunity

HideoNakarai¹ 

关键词: Endotoxemia;    low-grade inflammation;    metabolic diseases;    LPS binding protein;    adipocyte–macrophage interaction;   

DOI  :  10.1177/1753425910393370

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Chronic low-grade infection has been suggested to be associated with metabolic disorder such as diabetes. However, the molecular mechanism underlying this important association is largely unknown. The only clue established so far is that many subjects exhibit elevated levels of C-reactive protein as measured by highly sensitive assay. Here, we hypothesized that adipocyte–macrophage interaction plays a key role in amplifying such low grade infection to the level of influencing metabolic disorders. The presence of macrophages in abdominal adipose tissues was investigated by immunohistochemistry. To see whether molecules associated with acute phase protein, LPS signaling, and persistent recruitment of monocytes, are produced at higher amounts in adipocytes co-cultured with macrophages stimulated with low concentration of LPS (1 ng/ml), we measured serum amyloid A (SAA), LPS binding protein (LBP), soluble CD14 (sCD14), and RANTES levels in culture supernatant of co-cultures. Lastly, we investigated in vivo ef...

【 授权许可】

CC BY