Low susceptibility of NC/Nga mice to the lipopolysaccharide-mediated lethality with d-galactosamine sensitization and the involvement of fewer natural killer T cells:

Innate Immunity

NaokiKoide¹ 

关键词: NC/Nga mice;    lipopolysaccharide;    nitric oxide;    NKT cell;    α-galactosylceramide;   

DOI  :  10.1177/1753425910390400

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

The LPS-mediated lethality of NC/Nga mice, having fewer NKT cells, was examined by using d-galactosamine (d-GalN)-sensitization. The NC/Nga mice were not killed by a simultaneous administration of d-GalN and LPS whereas all C57BL/6 (B6) control mice were killed. The injection of d-GalN and LPS failed to elevate the levels of serum alanine aminotransferase and caspase 3 in the liver tissues of NC/Nga mice. Further, the nitric oxide (NO) level of the d-GalN- and LPS-injected NC/Nga mice was much lower than those of the B6 mice. The expression of an inducible NO synthase (iNOS) was significantly reduced in the livers of NC/Nga mice. However, there was no significant difference in LPS-induced TNF-α production between B6 mice and NC/Nga mice. The NC/Nga mice had an impaired expression of IFN-γ protein and mRNA in response to d-GalN and LPS. The pretreatment with α-galactosylceramide (α-GalCer), which activates Vα14+ NKT cells and induces the production of IFN-γ, rendered NC/Nga mice more susceptible to the LPS...

【 授权许可】

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