Сочетанная трансплантация гепатоцитов с мезенхимальными стволовыми клетками костного мозга на клеточных носителях из пористопроницаемого никелида титана

Acta Biomedica Scientifica

С. В. Гюнтер¹  О. В. Кокорев²  Г. Ц. Дамбаев²  В. Н. Ходоренко² 

[1] ФГБОУ ВО «Сибирский государственный медицинский университет» Минздрава России;ФГАОУ ВО «Национальный исследовательский Томский государственный университет»;ФГБОУ ВО «Сибирский государственный медицинский университет» Минздрава России

关键词: тканевая инженерия;    печёночная недостаточность;    трансплантация клеток;    клеточный носитель;    пористый никелид титана;   

DOI  :  10.29413/ABS.2018-3.3.30

学科分类：自然科学（综合）

来源: Scientific entre for Family Health and Human Reproduction Problems

【 摘 要 】

The aim of the research was to study of the therapeutic efficacy of combined transplantation of liver cells and mesenchymal stem cells of bone marrow (MSC) on TiNi-based alloy scaffolds of rats with chronic hepatitis. Materials and methods: Three groups of experiments were carried out on Wistar rats and intact rats (control) - I group; in the II group - chronic hepatitis; III group - cell-engineering designs with liver cells; in the IV group - cell-engineering designs with liver cells and BM MMSC. The activity of recovery processes was evaluated by using biochemical methods in dynamics on 10, 20, 30th days. Results. The optimal ratio of liver cells to MSC (5:1) was previously found in TiNi-based alloy scaffolds after transplantation and through co-cultivation of hepatocytes and MSC at different ratios. It was shown that recovery processes in the group with scaffolds transplanted into damaged livers were much more active if compared with those in the control group with chronic hepatitis. At the same time, in the group with combined transplantation on TiNi-based alloy scaffolds contributed to a more rapid normalization of liver enzyme indices compared with the group with transplantation of some hepatocytes on scaffolds. These data are confirmed by an increase in the life span of animals with combined hepatocytes and MSC transplantation on TiNi-based alloy scaffolds. Conclusions. A higher level and prolonged periods of liver regeneration during the transplantation of TiNi-based alloy scaffolds are due to the creation of biologically appropriate conditions for cells with certain ratio.

【 授权许可】

CC BY