Protein-bound polysaccharide-K induces IL-1β via TLR2 and NLRP3 inflammasome activation:

Innate Immunity

YiYang¹ 

关键词: TLR2;    NLRP3 inflammasome;    PSK;   

DOI  :  10.1177/1753425913513814

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Inflammasome activation has been shown to regulate both innate and adaptive immune responses. It is important to investigate whether immune-enhancing natural products can also activate inflammasome. The current study examined the potential of protein-bound polysaccharide-K (PSK), a hot water extract from Trametes versicolor, to activate inflammasome. Using THP-1 cells, we have demonstrated that PSK induces both pro-IL-1β and mature IL-1β in THP-1 cells in a caspase 1- and NLRP3-dependent manner. PSK also induces IL-1β and IL-18 in human PBMC. Cathepsin B is required for PSK-induced inflammasome activation as CA-074-Me, a cathepsin B inhibitor, significantly decreased PSK-induced IL-1β. PSK induces NLRP3 at both mRNA and protein level. Comparison of PSK-induced IL-1β in bone marrow-derived macrophages from wild type C57BL/6 mice, TLR2-/-, P2X7R-/- and NLRP3-/- mice demonstrated that PSK-induced IL-1β is dependent on both TLR2 and NLRP3. P2X7R is not required for PSK-induced inflammasome activation, but enh...

【 授权许可】

CC BY