In vivo induction of neutrophil extracellular traps by Mycobacterium tuberculosis in a guinea pig model:

Innate Immunity

GeorginaFilio-Rodríguez¹ 

关键词: In vivo;    neutrophils;    NETs;    Mycobacterium tuberculosis;    guinea pig;   

DOI  :  10.1177/1753425917732406

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

In 2004, a novel mechanism of cellular death, called ‘NETosis’, was described in neutrophils. This mechanism, different from necrosis and apoptosis, is characterized by the release of chromatin webs admixed with microbicidal granular proteins and peptides (NETs). NETs trap and kill a variety of microorganisms. Diverse microorganisms, including Mycobacterium tuberculosis, are NET inducers in vitro. The aim of this study was to examine whether M. tuberculosis can also induce NETs in vivo and if the NETs are bactericidal to the microorganism. Guinea pigs were intradermally inoculated with M. tuberculosis H37Rv, and the production of NETs was investigated at several time points thereafter. NETs were detected as early as 30 min post-inoculation and were clearly evident by 4 h post-inoculation. NETs produced in vivo contained DNA, myeloperoxidase, elastase, histones, ROS and acid-fast bacilli. Viable and heat-killed M. tuberculosis, as well as Mycobacterium bovis BCG were efficient NET inducers, as were unilame...

【 授权许可】

CC BY