Human Mesenchymal Cell Attachment, Growth and Biomineralization on Calcium-enriched Titania-polyester Coatings

AIMS Cell and Tissue Engineering

Nicholas Y. Hou¹ 

[1] 1 Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada2 Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 5C1, Canada

关键词: implants;    coatings;    titanium dioxide;    calcium;    mesenchymal cells;   

DOI  :  10.3934/celltissue.2017.2.64

学科分类：医学（综合）

来源: AIMS Press

【 摘 要 】

Titanium implant osseointegration can be enhanced by surface modifications that include hydroxyapatite from Ca3(PO4)2. However, CaO may provide more surface calcium (w/w) to induce cellular responses. Therefore, the purpose of this study was to compare responses to novel CaO and Ca3(PO4)2-enriched titania-polyester (PPC) nanocomposite coatings, which were created by an electrostatic ultrafine dry powder coating technique. EDX confirmed the presence of a base polymer scaffold, biocompatible titanium, and CaO or Ca3(PO4)2. SEM showed that human embryonic palatal mesenchymal cells (ATCC CRL-1486) had attached and spread out onto all surfaces within 24 hours. Cell attachment assays showed that there was a progressive increase in cell numbers with surface CaO incorporation (0–5%), such that the PPC + 5% CaO coatings supported the most cells. Furthermore, the PPC + 5% CaO had significantly more (P = 0.006) cells attached to their surfaces than the PPC + 5% CaP coatings and titanium controls, at 24 hours. The PPC + 5% CaO also had more cells that had proliferated on their surfaces over 72 hours, although these differences were not significant (P > 0.05). Similarly, MTT assays showed that the cells had sustained metabolic activity on all surfaces. Again, metabolic activities were highest on the PPC + 5% CaO, and they were significantly higher (P < 0.05) on allCaO-enriched surfaces (1/3/5% CaO) than on the PPC + 5% CaP. Subsequently, Alizarin Red-S staining detected the initiation of biomineralization within 2 weeks, and abundant mineral deposits after 4 weeks of growth on PPC + 5% CaO and PPC + 3% CaO. These nanocomposite coatings have shown that CaO enrichments may provide a heightened cell response when compared to conventional hydroxyapatite.

【 授权许可】

CC BY