Human papillomavirus type 16 (HPV16) DNA copy number in oral rinse samples from oral cavity cancer patients:

Translational Research in Oral Oncology

HideoShigeishi¹ 

关键词: HPV16;    viral copy number;    oral cavity cancer;   

DOI  :  10.1177/2057178X18774683

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Objectives:The objective of this study is to examine the human papillomavirus type 16 (HPV16) DNA copy number in the oral cavity of cancer patients using oral rinse samples.Methods:We analyzed the HPV16 DNA copy number of oral rinse samples in 13 primary oral cavity cancer patients (mean age 67.8 years, range 48–84 years) who visited the Department of Oral and Maxillofacial Reconstructive Surgery of Hiroshima University Hospital (Hiroshima, Japan). The 13 oral cavity cancers included 6 carcinomas in situ and 7 squamous cell carcinomas. Real-time PCR analysis was performed to examine the number of HPV16 E6 viral copies in oral rinse samples. Additionally, the HPV16 integration status was investigated using the real-time PCR findings for HPV16 E2 genes in HPV16 E6-positive oral rinse samples.Results:HPV16 E6 copy numbers above the detection limit in a standard curve for HPV16 E6 DNA were assessed as HPV16 positive in oral rinse samples from 6 of 13 patients. The average number of HPV16 E6 viral copies was 1...

【 授权许可】

CC BY