In-Vivo Test of Spirulina sp as Inducer of β-Actin In Cantang Grouper (Epinephelus fuscoguttatus-lanceolatus) Infected by Viral Nervous Necrosis

JFMR-Journal of Fisheries and Marine Research

Yovan Endik Irawanto¹  Uun Yanuhar¹  Andi Kurniawan¹ 

[1] Brawijaya University

关键词: Epinephelus sp;    Spirulina sp;    Viral Nervous Necrosis;    β-actin;   

DOI  :  10.21776/ub.jfmr.2018.002.03.11

学科分类：农业科学（综合）

来源: University of Brawijaya

【 摘 要 】

Cantang grouper fish ( Epinephelus fuscoguttatus-lanceolatus ) is a result of hybridized fish between a female of tiger grouper fish ( Epinephelus fuscoguttatus ) and male of kertang grouper ( Ephinephelus lanceolatus ). In the development of cultivation, there are many problems, one of them infected with the class of virus Nodaviridae, namely Viral Nervous Necrosis (VNN). Fish had a defense against cellular immunity against the VNN virus by β-actin. The aims of this research to explore the crude extract ofSpirulinasp as a β-actin inducer for the anti-inflammatory immune system in grouper fish against VNN attack. The method used in this research is experiment methods.  Crude extracts ofSpirulinasp (33 μg/ml) were conducted by feeding orally to groupers, and VNN infections were conducted by feeding the already positive VNN meat. Detection of VNN using RT-PCR, however β-actin detection using PCR, and IHC in the organ of Cantang grouper fish. The results showed that the percentage of DAB value of control fish (14.0%), fish treated withSpirulinasp (25.7%), fish treated with VNN (31.9%), and fish treated withSpirulinasp extract and VNN infection (32.4%). The percentage of DAB values were indicated by the detection of the target gene β-actin. Immunity in fish increases with the addition ofSpirulinasp. The increased β-actin expression may also be used as an indicator of a grouper's body defense against VNN infection.

【 授权许可】

CC BY-NC