Brief on -Hyphenated Methods of HPLC for Determining the Presence of Solutes.

Chronicles of Pharmaceutical Science

Mohamad Taleuzzaman*¹ 

[1] Glocal School of Pharmacy, Glocal University, Mirzapur Pole, Saharanpur, 247121. U.P

关键词: Hyphenated;    Chromatography;    Detector;    Particle size;    Quantitive analysis;   

DOI  :  

学科分类：医学（综合）

来源: Scientia Ricerca

【 摘 要 】

HPLC is the tool in liquid chromatography is unique because of particle size, smaller particle in the stationary phase, increase efficiency of a separation. However, if the particles are made smaller, capillary action increases and it becomes more difficult to drain the column under gravity. For quantitative analysis different types of detector is used in conjunction with HPLC which give precise and accurate result and it is apply according to the nature of the substance. Various types of detectors used in HPLC are mass spectrometry, infrared spectroscopy, visible spectroscopy, ultraviolet spectroscopy, fluorescence spectroscopy, nuclear magnetic resonance, conductivity measurement, and refractive index measurement. Each detector has its assets, limitations and sample types for which it is most effective. The recent development of the so-called hyphenated techniques has improved the ability to separate and identify multiple entities within a mixture.

【 授权许可】

CC BY