JNCI Cancer Spectrum | |
Major Impact of Sampling Methodology on Gene Expression in Estrogen Receptor–Positive Breast Cancer | |
Dowsett, Mitch1  López-Knowles, Elena1  Gao, Qiong1  Sidhu, Kally1  Martin, Lesley-Ann2  Ribas, Ricardo3  Morden, James3  Robertson, John3  Bliss, Judith M4  Evans, Abigail5  Evans, David6  Skene, Anthony6  Dodson, Andrew6  U Cheang, Maggie Chon6  Martins, Vera6  Holcombe, Chris7  Mallon, Elizabeth8  Smith, Ian9  | |
[1] Breast Cancer Now Research Centre, The Institute of Cancer Research, London, UK;Breast Unit, Royal Marsden Hospital, London, UK;Clinical Trials and Statistics Unit, The Institute of Cancer Research, London, UK;Poole General Hospital, Dorset, UK;Queen Elizabeth University Hospital Glasgow, Govan, UK;Ralph Lauren Centre for Breast Cancer Research;Royal Bournemouth Hospital, Bournemouth, UK;Royal Liverpool University Hospital, Liverpool, UK;University of Nottingham, Nottingham, UK | |
关键词: gene expression; surgical procedures, operative; breast cancer; biopsy; aromatase inhibitors; perceptual masking; | |
DOI : 10.1093/jncics/pky005 | |
学科分类:肿瘤学 | |
来源: Oxford University Press | |
【 摘 要 】
To investigate the impact of sampling methodology on gene expression data from primary estrogen receptor–positive (ER+) breast cancer biopsies, global gene expression was measured in core-cut biopsies at baseline and surgery from patients randomly assigned to receive either two weeks of presurgical aromatase inhibitor (AI; n = 157) or no presurgical treatment (n = 56). Those genes most markedly altered in the AI group (eg, FOS, DUSP1, RGS1, FOSB) were similarly altered in the no treatment group; some widely investigated genes that were apparently unaffected in the AI group (eg, MYC) were counter-altered in the control group, masking actual AI-dependent changes. In the absence of a control group, these artefactual changes would likely lead to the most affected genes being the erroneous focus of research. The findings are likely relevant to all archival collections of ER+ breast cancer.
【 授权许可】
CC BY-NC-ND
【 预 览 】
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RO201904026103770ZK.pdf | 797KB | download |