Major Impact of Sampling Methodology on Gene Expression in Estrogen Receptor–Positive Breast Cancer

JNCI Cancer Spectrum

Dowsett, Mitch¹  López-Knowles, Elena¹  Gao, Qiong¹  Sidhu, Kally¹  Martin, Lesley-Ann²  Ribas, Ricardo³  Morden, James³  Robertson, John³  Bliss, Judith M⁴  Evans, Abigail⁵  Evans, David⁶  Skene, Anthony⁶  Dodson, Andrew⁶  U Cheang, Maggie Chon⁶  Martins, Vera⁶  Holcombe, Chris⁷  Mallon, Elizabeth⁸  Smith, Ian⁹ 

[1] Breast Cancer Now Research Centre, The Institute of Cancer Research, London, UK;Breast Unit, Royal Marsden Hospital, London, UK;Clinical Trials and Statistics Unit, The Institute of Cancer Research, London, UK;Poole General Hospital, Dorset, UK;Queen Elizabeth University Hospital Glasgow, Govan, UK;Ralph Lauren Centre for Breast Cancer Research;Royal Bournemouth Hospital, Bournemouth, UK;Royal Liverpool University Hospital, Liverpool, UK;University of Nottingham, Nottingham, UK

关键词: gene expression;    surgical procedures, operative;    breast cancer;    biopsy;    aromatase inhibitors;    perceptual masking;   

DOI  :  10.1093/jncics/pky005

学科分类：肿瘤学

来源: Oxford University Press

【 摘 要 】

To investigate the impact of sampling methodology on gene expression data from primary estrogen receptor–positive (ER+) breast cancer biopsies, global gene expression was measured in core-cut biopsies at baseline and surgery from patients randomly assigned to receive either two weeks of presurgical aromatase inhibitor (AI; n = 157) or no presurgical treatment (n = 56). Those genes most markedly altered in the AI group (eg, FOS, DUSP1, RGS1, FOSB) were similarly altered in the no treatment group; some widely investigated genes that were apparently unaffected in the AI group (eg, MYC) were counter-altered in the control group, masking actual AI-dependent changes. In the absence of a control group, these artefactual changes would likely lead to the most affected genes being the erroneous focus of research. The findings are likely relevant to all archival collections of ER+ breast cancer.

【 授权许可】

CC BY-NC-ND