The role of MicroRNAs miR-200b and miR-200c in TLR4 signaling and NF-κB activation:

Innate Immunity

Erik BWendlandt¹ 

关键词: Macrophage;    microRNAs;    Toll-like receptor;    gene expression;    pro-inflammatory response;   

DOI  :  10.1177/1753425912443903

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Recognition of microbial products by members of the Toll-like receptor (TLR) family initiates intracellular signaling cascades that result in NF-κB activation and subsequent production of inflammatory cytokines. We explored the potential roles of microRNAs (miRNAs) in regulating TLR pathways. A target analysis approach to the TLR4 pathway adaptor molecules identified several putative targets of miR-200a, miR-200b and miR-200c. miRNA mimics were co-transfected with a NF-κB activity reporter plasmid into HEK293 cells stably expressing TLR4 (HEK293-TLR4). Mimics of both miR-200b and miR-200c, but not miR-200a, decreased NF-κB reporter activity in either untreated cells or in cells treated with endotoxin:MD2 as a TLR4 agonist. Transfection of HEK293-TLR4 cells with miR-200b or miR-200c significantly decreased expression of MyD88, whereas TLR4, IRAK-1 and TRAF-6 mRNAs were unaffected. When miR-200b or miR-200c mimics were transfected into the differentiated monocytic THP-1 cell line, the abundance of MyD88 tra...

【 授权许可】

CC BY