Innate immunity of surfactant proteins A and D in urinary tract infection with uropathogenic Escherichia coli:

Innate Immunity

FengqiHu¹ 

关键词: Innate immunity;    surfactant protein A;    surfactant protein D;    urinary tract infection;    uropathogenic Escherichia coli;   

DOI  :  10.1177/1753425915609973

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

To investigate the effects of surfactant proteins A and D (SP-A and SP-D, respectively) in urinary tract infection (UTI), SP-A and SP-D double knockout (SP-A/D KO) and wild type (WT) C57BL/6 female mice were infected with uropathogenic Escherichia coli by intravesical inoculation. Compared with WT mice SP-A/D KO mice showed increased susceptibility to UTI, as evidenced by higher bacterial CFU, more infiltrating neutrophils and severe pathological changes. Keratinocyte-derived chemokine increased in the kidney of WT mice but not in SP-A/D KO mice 24 h post-infection. Compared with control, the level of IL-17 was elevated in the kidney of infected WT and SP-A/D KO mice and the level of IL-17 was higher in the infected SP-A/D KO mice than in infected WT mice 24 and 48 h post-infection. The basal level of p38 MAPK phosphorylation in SP-A/D KO mice was higher than in WT mice. The phosphorylated p38 level was elevated in the kidney of WT mice post infection but not in SP-A/D KO mice. Furthermore, in vitro growt...

【 授权许可】

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