A new experimental murine model for lipopolysaccharide-mediated lethal shock with lung injury:

Innate Immunity

TakashiYokochi¹ 

关键词: LPS;    alpha-galactosylceramide;    interferon-gamma;    endotoxic shock;    NKT cells;    interleukin-4;    experimental model;   

DOI  :  10.1177/1753425911410236

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

We have recently established a new experimental murine model for lipopolysaccharide (LPS)-mediated lethal shock with lung-specific injury. Severe lung injury is induced by administration of LPS into α-galactosylceramide (α-GalCer)-sensitized mice; the mice died with acute lung injury and respiratory distress within 24 h. α-GalCer activates natural killer T (NKT) cells in the lungs and liver, and induces the production of interferon (IFN)-γ. However, IFN-γ signaling is only triggered in the lungs and makes them susceptible to LPS. On the other hand, IFN-γ signaling is inhibited in liver and results in few hepatic lesions. Unlike liver NKT cells, lung NKT cells fail to produce interleukin (IL)-4, which down-regulates the IFN-γ signaling, in response to α-GalCer. The differential cytokine profile between lung and liver NKT cells may lead to organ-specific lung lesions. The experimental system using α-GalCer sensitization could be a useful experimental model for clinical endotoxic or septic shock as it presen...

【 授权许可】

CC BY