Improved Cellular Specificity of Plasmonic Nanobubbles versus Nanoparticles in Heterogeneous Cell Systems

PLoS One

Pamela E. Constantinou¹  Ekaterina Y. Lukianova-Hleb¹  Daniel S. Wagner¹  Dmitri O. Lapotko¹  Daniel D. Carson¹  Brian P. Danysh¹  Derek L. Shenefelt¹  Mary C. Farach-Carson¹  Xiaoyang Ren²  Xiangwei Wu²  Vladimir A. Kulchitsky³ 

[1] Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, United States of America;Department of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America;Institute of Physiology, National Academy of Science of Belarus, Minsk, Belarus

关键词: Lasers;    Nanoparticles;    Bionanotechnology;    Endocytosis;    Fluorescence imaging;    Vapors;    Cancer treatment;    Vapor pressure;   

DOI  :  10.1371/journal.pone.0034537

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

The limited specificity of nanoparticle (NP) uptake by target cells associated with a disease is one of the principal challenges of nanomedicine. Using the threshold mechanism of plasmonic nanobubble (PNB) generation and enhanced accumulation and clustering of gold nanoparticles in target cells, we increased the specificity of PNB generation and detection in target versus non-target cells by more than one order of magnitude compared to the specificity of NP uptake by the same cells. This improved cellular specificity of PNBs was demonstrated in six different cell models representing diverse molecular targets such as epidermal growth factor receptor, CD3 receptor, prostate specific membrane antigen and mucin molecule MUC1. Thus PNBs may be a universal method and nano-agent that overcome the problem of non-specific uptake of NPs by non-target cells and improve the specificity of NP-based diagnostics, therapeutics and theranostics at the cell level.

【 授权许可】

CC BY