Biocontrol of wilt-nematode complex infecting gerbera by Bacillus subtilis under protected cultivation

Egyptian Journal of Biological Pest Control

E. I. Jonathan¹  K. Sankari Meena¹  T. Raguchander²  SA. Ramyabharathi²  G. Karthikeyan²  L. Rajendran² 

[1] Department of Nematology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, India;Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, India

关键词: Bacillus subtilis Bbv 57;    Surfactin;    Iturin;    ß-Glucanase;    Fusarium nematode complex;    Greenhouse;   

DOI  :  10.1186/s41938-018-0027-2

学科分类：动物科学

来源: Springer

【 摘 要 】

A strong antibiotic producer, Bacillus subtilis strain Bbv 57 (KF718836), has been utilized for the management of wilt-nematode complex (Fusarium oxysporum f. sp. gerberae, Meloidogyne incognita) in gerbera under greenhouse conditions in the Department of Floriculture, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India. The strain strongly inhibited F. oxysporum f. sp. gerberae (KM523669) mycelial growth to an extent of 44.33 and 63.33%, at 10 and 100 μl, of culture filtrate, respectively. Further, the culture filtrate at 100% concentration exerted lethal effect on nematode eggs (7.00 hatched) and juveniles (87% mortality) compared to control. The analysis of TLC revealed that Bbv 57 showed the cyclic antimicrobial peptides surfactin and iturin that were confirmed by PCR. Strain Bbv 57 was able to produce antifungal and anti-nematicidal activity with reduced wilt incidence (15.33%) and thus holds a great potential for use in the biocontrol of Fusarium wilt-root-knot nematode disease complex in gerbera under greenhouse conditions.

【 授权许可】

CC BY