Association between Inflammatory Infiltrates and Isolated Monosomy 22/del(22q) in Meningiomas

PLoS One

Álvaro Otero¹  María del Carmen García Macias¹  Maria Dolores Tabernero²  Jesús María Gonçalves³  Pablo Sousa³  Cristina Teodósio⁴  Javier Ortiz⁴  Patrícia Henriques Domingues⁵  Alberto Orfao⁶  Ana Belén Nieto⁷  María Celeste Lopes⁷  Catarina de Oliveira⁸ 

[1] CSIC/USAL;CSIC/USAL), Salamanca, Spain;IBSAL) and Department of Medicine, University of Salamanca, Salamanca, Spain;Centre for Cancer Research (CIC-IBMCC;Centre for Neurosciences and Cell Biology and Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal;Molecular Pathology Service, Centre for Cancer Research (CIC-IBMCC;Neurosurgery Service, University Hospital of Salamanca, Salamanca, Spain;Pathology Service, University Hospital of Salamanca, Salamanca, Spain

关键词: Meningioma;    Cytogenetics;    Inflammation;    Macrophages;    Karyotypes;    Lymphocytes;    Immune cells;    Toll-like receptors;   

DOI  :  10.1371/journal.pone.0074798

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Meningiomas contain highly variable levels of infiltrating tissue macrophages (TiMa) and other immune cells. In this study we investigated the potential association between the number and immunophenotype of inflammatory and other immune cells infiltrating the tumor as evaluated by multiparameter flow cytometry, and the clinico-biological, cytogenetic and gene expression profile (GEP) of 75 meningioma patients. Overall, our results showed a close association between the amount and cellular composition of the inflammatory and other immune cell infiltrates and the cytogenetic profile of the tumors. Notably, tumors with isolated monosomy 22/del(22q) showed greater numbers of TiMa, NK cells and (recently)-activated CD69+ lymphocytes versus meningiomas with diploid and complex karyotypes. In addition, in the former cytogenetic subgroup of meningiomas, tumor-infiltrating TiMa also showed a more activated and functionally mature phenotype, as reflected by a greater fraction of CD69+, CD63+, CD16+ and CD33+ cells. GEP at the mRNA level showed a unique GEP among meningiomas with an isolated monosomy 22/del(22q) versus all other cases, which consisted of increased expression of genes involved in inflammatory/immune response, associated with an M1 TiMa phenotype. Altogether, these results suggest that loss of expression of specific genes coded in chromosome 22 (e.g. MIF) is closely associated with an increased homing and potentially also anti-tumoral effect of TiMa, which could contribute to explain the better outcome of this specific good-prognosis cytogenetic subgroup of meningiomas.

【 授权许可】

CC BY