Genome-Wide Association Studies Identifies Seven Major Regions Responsible for Iron Deficiency Chlorosis in Soybean (Glycine max)

PLoS One

Rian K. Lee¹  Phillip E. McClean¹  Jay R. Goos²  Sujan Mamidi² 

[1] Department of Plant Sciences, North Dakota State University, Fargo, North Dakota, United States of America;Genomics and Bioinformatics Program, North Dakota State University, Fargo, North Dakota, United States of America

关键词: Soybean;    Iron deficiency;    Genome-wide association studies;    Alleles;    Leaves;    Nitrates;    Quantitative trait loci;    Molecular genetics;   

DOI  :  10.1371/journal.pone.0107469

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Iron deficiency chlorosis (IDC) is a yield limiting problem in soybean (Glycine max (L.) Merr) production regions with calcareous soils. Genome-wide association study (GWAS) was performed using a high density SNP map to discover significant markers, QTL and candidate genes associated with IDC trait variation. A stepwise regression model included eight markers after considering LD between markers, and identified seven major effect QTL on seven chromosomes. Twelve candidate genes known to be associated with iron metabolism mapped near these QTL supporting the polygenic nature of IDC. A non-synonymous substitution with the highest significance in a major QTL region suggests soybean orthologs of FRE1 on Gm03 is a major gene responsible for trait variation. NAS3, a gene that encodes the enzyme nicotianamine synthase which synthesizes the iron chelator nicotianamine also maps to the same QTL region. Disease resistant genes also map to the major QTL, supporting the hypothesis that pathogens compete with the plant for Fe and increase iron deficiency. The markers and the allelic combinations identified here can be further used for marker assisted selection.

【 授权许可】

CC BY