α2,3-Sialyltransferase ST3Gal III Modulates Pancreatic Cancer Cell Motility and Adhesion In Vitro and Enhances Its Metastatic Potential In Vivo

PLoS One

Lluís Pagès¹  Rosa Peracaula¹  Marta Pérez-Garay¹  Rafael de Llorens¹  Carme de Bolòs²  Fernando Vidal-Vanaclocha³  Beatriz Arteta³ 

[1] Biochemistry and Molecular Biology Unit, Department of Biology, University of Girona, Girona, Spain;Cancer Research Program, IMIM-Hospital del Mar, Barcelona, Spain;Department of Cell Biology and Histology, School of Medicine and Dentistry, Basque Country University, Leioa, Spain

关键词: Cloning;    Metastasis;    Mouse models;    Cell migration;    Pancreatic cancer;    Adenocarcinoma cells;    Cancer cell migration;    Cell binding;   

DOI  :  10.1371/journal.pone.0012524

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Background Cell surface sialylation is emerging as an important feature of cancer cell metastasis. Sialyltransferase expression has been reported to be altered in tumours and may account for the formation of sialylated tumour antigens. We have focused on the influence of alpha-2,3-sialyltransferase ST3Gal III in key steps of the pancreatic tumorigenic process.Methodology/Principal Findings ST3Gal III overexpressing pancreatic adenocarcinoma cell lines Capan-1 and MDAPanc-28 were generated. They showed an increase of the tumour associated antigen sialyl-Lewisx. The transfectants' E-selectin binding capacity was proportional to cell surface sialyl-Lewisx levels. Cellular migration positively correlated with ST3Gal III and sialyl-Lewisx levels. Moreover, intrasplenic injection of the ST3Gal III transfected cells into athymic nude mice showed a decrease in survival and higher metastasis formation when compared to the mock cells.Conclusion In summary, the overexpression of ST3Gal III in these pancreatic adenocarcinoma cell lines underlines the role of this enzyme and its product in key steps of tumour progression such as adhesion, migration and metastasis formation.

【 授权许可】

CC BY