Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells:

Innate Immunity

Paola LSmaldini¹ 

关键词: Down-regulation;    Gordonia bronchialis;    NF-κB;    epithelial cells;    CCL20;    inflammation;   

DOI  :  10.1177/1753425913503577

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

The immunomodulatory power of heat-killed Gordonia bronchialis was studied on gut epithelial cells activated with pro-inflammatory stimuli (flagellin, TNF-α or IL-1β). Light emission of luciferase-transfected epithelial cells and mRNA expression of IL-1β, TNF-α, IL-6, CCL20, IL-8 and MCP-1 were measured. NF-κB activation was assessed by immunofluorescence and immunoblotting, and induction of reactive oxygen species (ROS) was evaluated. In vivo inhibitory properties of G. bronchialis were studied with ligated intestinal loop assay and in a mouse model of food allergy. G. bronchialis promoted the down-regulation of the expression of CCL20 and IL-1β on activated epithelial cells in a dose-dependent manner. A concomitant blocking of nuclear p65 translocation with increased production of ROS was found. In vivo experiments confirmed the inhibition of CCL20 expression and the suppression of IgE sensitization and hypersensitivity symptoms in the food allergy mouse model. In conclusion, heat-killed G. bronchialis ...

【 授权许可】

CC BY