Analysis of the Caenorhabditis elegans innate immune response to Coxiella burnetii:

Innate Immunity

James MBattisti¹ 

关键词: Coxiella burnetii;    Caenorhabditis elegans;    human pathogenic bacteria;    immunity;    Q fever;   

DOI  :  10.1177/1753425916679255

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

The nematode Caenorhabditis elegans is well established as a system for characterization and discovery of molecular mechanisms mediating microbe-specific inducible innate immune responses to human pathogens. Coxiella burnetii is an obligate intracellular bacterium that causes a flu-like syndrome in humans (Q fever), as well as abortions in domesticated livestock, worldwide. Initially, when wild type C. elegans (N2 strain) was exposed to mCherry-expressing C. burnetii (CCB) a number of overt pathological manifestations resulted, including intestinal distension, deformed anal region and a decreased lifespan. However, nematodes fed autoclave-killed CCB did not exhibit these symptoms. Although vertebrates detect C. burnetii via TLRs, pathologies in tol-1(–) mutant nematodes were indistinguishable from N2, and indicate nematodes do not employ this orthologue for detection of C. burnetii. sek-1(–) MAP kinase mutant nematodes succumbed to infection faster, suggesting that this signaling pathway plays a role in i...

【 授权许可】

CC BY