Contribution of Drosophila TRPA1 to Metabolism

PLoS One

Do Yup Lee¹  Yunjung Kim¹  Youngseok Lee¹  Jung-Eun Lee¹  Kyoung Heon Kim² 

[1]Department of Bio and Fermentation Convergence Technology, BK21 PLUS project, Kookmin University, Seoul 02707, Korea

[2]Department of Biotechnology, Graduate School, Korea University, Seoul, Korea

关键词: Metabolites;    Methionine;    Metabolic pathways;    Drosophila melanogaster;    Metabolic networks;    Carbohydrate metabolism;    Fatty acids;    Amino acid metabolism;   

DOI  :  10.1371/journal.pone.0152935

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Transient receptor potential (TRP) cation channels are highly conserved in humans and insects. Some of these channels are expressed in internal organs and their functions remain incompletely understood. By direct knock-in of the GAL4 gene into the trpA1 locus in Drosophila, we identified the expression of this gene in the subesophageal ganglion (SOGs) region. In addition, the neurites present in the dorsal posterior region as well as the drosophila insulin-like peptide 2 (dILP2)-positive neurons send signals to the SOGs. The signal is sent to the crop, which is an enlarged organ of the esophagus and functions as a storage place for food in the digestive system. To systematically investigate the role of TRPA1 in metabolism, we applied non-targeted metabolite profiling analysis together with gas-chromatography/time-of-flight mass spectrometry, with an aim to identify a wide range of primary metabolites. We effectively captured distinctive metabolomic phenotypes and identified specific metabolic dysregulation triggered by TRPA1 mutation based on reconstructed metabolic network analysis. Primarily, the network analysis pinpointed the simultaneous down-regulation of intermediates in the methionine salvation pathway, in contrast to the synchronized up-regulation of a range of free fatty acids. The gene dosage-dependent dynamics of metabolite levels among wild-type, hetero- and homozygous mutants, and their coordinated metabolic modulation under multiple gene settings across five different genotypes confirmed the direct linkages of TRPA1 to metabolism.

【 授权许可】

CC BY