The role of DNA sensing and innate immune receptor TLR9 in otitis media:

Innate Immunity

AnkeLeichtle¹ 

关键词: CpG DNA;    Haemophilus influenzae;    otitis media;    pathogen DNA sensing;    Toll-like receptor;   

DOI  :  10.1177/1753425910393539

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Otitis media (OM), a common infectious disease in children, is associated with bacterial middle ear (ME) infection. Toll-like receptors (TLRs) are important mediators of innate immune responses, and TLR9 specifically recognizes the unmethylated cytidine-phosphate-guanosine (CpG) motifs in bacterial DNA. Additional sensors of foreign DNA have recently been identified. The role of DNA sensing and TLR9 was investigated in a murine model of OM induced by non-typeable Haemophilus influenzae (NTHi). Expression of genes related to DNA-sensing pathways involved in innate immunity was assessed via DNA microarray, qPCR and immunohistochemistry. Middle ear responses to NTHi were examined in wild-type and TLR9−/− mice by histopathology and bacterial culture. Expression of TLR9 signaling genes was modestly up-regulated during OM, as was TLR9 protein in both ME mucosal cells and infiltrating leukocytes. However, genes known to be regulated by CpG DNA were dramatically up-regulated, as were genes involved in DNA sensing...

【 授权许可】

CC BY