Low-dose polymyxin: an option for therapy of Gram-negative sepsis:

Innate Immunity

StephanHarm¹ 

关键词: Polymyxin;    lipopolysaccharide;    sepsis;    Inflammation;    cytokines;    endotoxin;   

DOI  :  10.1177/1753425916639120

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Endotoxins are the major components of the outer membrane of most Gram-negative bacteria and are one of the main targets in inflammatory diseases. The presence of endotoxins in blood can provoke septic shock in case of pronounced immune response. Here we show in vitro inactivation of endotoxins by polymyxin B (PMB). The inflammatory activity of the LPS–PMB complex in blood was examined in vitro in freshly drawn blood samples. Plasma protein binding of PMB was determined by ultracentrifugation using membranes with different molecular cut-offs, and PMB clearance during dialysis was calculated after in vitro experiments using the AV1000S filter. The formed LPS–PMB complex has lower inflammatory activity in blood, which results in highly reduced cytokine secretion. According to in vitro measurements, the appropriate plasma level of PMB for LPS inactivation is between 100 and 200 ng/ml. Furthermore, the combination of cytokine removal by adsorbent treatment with LPS inactivation by PMB dosage leads to strong s...

【 授权许可】

CC BY