Resveratrol inhibits LPS-induced epithelial-mesenchymal transition in mouse melanoma model:

Innate Immunity

Man-ChinChen¹ 

关键词: Resveratrol;    lipopolysaccharide;    epithelial to mesenchymal transition;    metastasis;    NF-κB;   

DOI  :  10.1177/1753425912436589

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Epithelial to mesenchymal transition (EMT) has been linked to metastasis. Resveratrol exhibits potential antitumor activities; however, the inhibitory effects of resveratrol on the EMT of melanoma have not been demonstrated. Here, a new role for LPS in promoting EMT is described. LPS-induced EMT was identified by examining the markers of EMT. To assess the activation of NF-κB signal transduction pathway, we performed a reporter assay by using tumor cells transfected with the luciferase gene under the control of NF-κB response elements. The antitumor effects of resveratrol were evaluated in an experimental mouse metastasis tumor model. LPS increased N-cadherin and Snail expression and decreased zonula occludens-1 expression in a dose- and time-dependent manner. Meanwhile, LPS stimulated cell migration through activation of TLR4/NF-κB signal pathway. LPS-induced EMT is critical for inflammation-initiated metastasis. Nuclear translocation and transcriptional activity of p65 NF-κB, an important inducer of EMT...

【 授权许可】

CC BY