Anti-inflammatory effects of prunin on UVB-irradiated human keratinocytes

Biomedical Dermatology

Eun Ju Na¹  Ji Young Ryu² 

[1]Department of Biological Engineering, Konkuk University, Seoul, Republic of Korea

[2]Halla University, Wonju-si, Republic of Korea

关键词: Prunin;    HaCaT keratinocyte;    Anti-inflammation;    Reactive oxygen species;    Ultraviolet B;   

DOI  :  10.1186/s41702-018-0024-9

学科分类：皮肤病学

来源: Springer

【 摘 要 】

The flavonoid prunin is a flavanone glycoside found in cherry trees, including the flowering cherry Prunus yedoensis Matsumura (Rosacea). Although this compound has been studied for its antioxidant, anti-bacterial, and blood-sugar-lowering effects, no studies address its use in cosmetics. This study investigates whether prunin exhibits anti-inflammatory effects in cells exposed to ultraviolet B (UVB) radiation. The effects of prunin were assessed by measuring cell viability using the water-soluble tetrazolium salt-1 assay and free radical damage using the dichlorofluorescein diacetate assay as well as by quantitative real-time PCR. UVB-induced decrease in cell viability diminished by pretreatment with prunin in a concentration-dependent manner. Intracellular reactive oxygen species (ROS) quantitative analysis revealed that the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), which is associated with the inflammatory response, and mRNA expression of interleukin-6 (IL-6), interleukin-8 (IL-8), cyclooxygenase-2 (COX2), tumor necrosis factor-alpha (TNF-α), and protease-activated receptor (PAR2) decreased with prunin pretreatment in a concentration-dependent manner. Prunin increased the survival rate of UVB-treated human keratinocyte HaCaT cells. Prunin protected the HaCaT cells, eliminated ROS, and demonstrated anti-inflammatory effects. Thus, prunin is worthy of investigation for use as a cosmetic ingredient that protects the skin and has anti-inflammatory effects.

【 授权许可】

CC BY