Distribution of Neuropeptides in the Synovium of Charcot Neuroarthropathy:

Foot & Ankle Orthopaedics

Lew C.Schon¹ 

关键词: synovium;    neuropeptide;    immunohistochemistry;    neuroarthropathy;    Charcot;   

DOI  :  10.1177/2473011416S00162

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Category:Basic Sciences/BiologicsIntroduction/Purpose:The pathology of Charcot neuroarthropathy (CNA) presents inflammation, and bone and cartilage destruction. It is still unclear how the neuropathic signals lead to joint inflammation and destruction. Neuropeptides are secreted by neurons and involve in the regulation of inflammation. Synovium is richly innervated and known to play a critical role in the pathology of inflammatory arthritis. This study is designed to investigate the distribution of several neuropeptides, such as vasoactive intestinal peptide (VIP), substance P (SP) and calcitonin gene-related peptide (CGRP), in CNA synovium, in comparison with non-CNA synovium, with immunohistochemistry.Methods:Synovial samples were collected from CNA patients (n=3) and patients with osteoarthritis and intraarticular fracture (non-CNA; n=3), during foot and ankle surgery (approved by IRB). The tissue samples were fixed with 4% paraformaldehyde, embedded in O.T.C media and sectioned with a cryostat. From e...

【 授权许可】

CC BY