Cigarette smoke condensate and individual constituents modulate DNA methyltransferase expression in human liver cells:

SAGE Open Medicine

YongmeiXiao¹ 

关键词: Cigarette smoke condensate;    DNA methyltransferase;    human liver cells;    nicotine;   

DOI  :  10.1177/2050312115578317

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Objectives:Previous studies found higher expression levels of DNA methyltransferase 1 in liver samples from smokers compared to those from non-smokers. In contrast, expression levels of DNA methyltransferase 3a and DNA methyltransferase 3b were similar in smokers and non-smokers. This study extends these studies to establish a causal linkage to cigarette smoke exposure by examining whether DNA methyltransferase expression is modulated by cigarette smoke condensate.Methods:These experiments were conducted in an in vitro system using HepG2 human liver cells. The dose range of cigarette smoke condensate was 0.1–120 µg/mL. The duration of exposure was up to 72 h.Results:In a 24-h exposure, DNA methyltransferase 1 expression was found to increase significantly in a dose-dependent manner (greater than threefold at 100 µg/mL cigarette smoke condensate). Expression levels of DNA methyltransferase 3a and DNA methyltransferase 3b were, however, not affected under these conditions. The effect of two cigarette consti...

【 授权许可】

CC BY