LPS challenge increased intestinal permeability, disrupted mitochondrial function and triggered mitophagy of piglets:

Innate Immunity

ShutingCao¹ 

关键词: LPS;    intestinal injury;    mitochondrial function;    mitophagy;    piglets;   

DOI  :  10.1177/1753425918769372

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Here we investigated the influence of LPS-induced gut injury on antioxidant homeostasis, mitochondrial (mt) function and the level of mitophagy in piglets. The results showed that LPS-induced intestinal injury decreased the transepithelial electrical resistance, increased the paracellular permeability of F1TC dextran 4 kDa, and decreased the expression of claudin-1, occludin and zonula occludens-1 in the jejunum compared with the control group. LPS decreased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and increased the content of malondialdehyde in the jejunum. Meanwhile, the expression of SOD-related genes (Cu/Zn-SOD, Mn-SOD) and GSH-Px-related genes (GPX-1, GPX-4) declined in LPS-challenged pigs compared with the control. LPS also increased TNF-α, IL-6, IL-8 and IL-1β mRNA expression. LPS induced mt dysfunction, as demonstrated by increased reactive oxygen species production and decreased membrane potential of intestinal mitochondria, intestinal content of mt DNA an...

【 授权许可】

CC BY