Soluble human TLR2 ectodomain binds diacylglycerol from microbial lipopeptides and glycolipids:

Innate Immunity

Maximiliano JJiménez-Dalmaroni¹ 

关键词: Diacylglycerol lig;    s;    lipoarabinomannan;    lipoteichoic acid;    FSL-1;    TLR2;   

DOI  :  10.1177/1753425914524077

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

TLRs are key innate immune receptors that recognize conserved features of biological molecules that are found in microbes. In particular, TLR2 has been reported to be activated by different kinds of microbial ligands. To advance our understanding of the interaction of TLR2 with its ligands, the recombinant human TLR2 ectodomain (hTLR2ED) was expressed using a baculovirus/insect cell expression system and its biochemical, as well as ligand binding, properties were investigated. The hTLR2ED binds synthetic bacterial and mycoplasmal lipopeptides, lipoteichoic acid from Staphylococcus aureus, and synthetic lipoarabinomannan precursors from Mycobacterium at extracellular physiological conditions, in the absence of its co-receptors TLR1 and TLR6. We also determined that lipopeptides and glycolipids cannot bind simultaneously to hTLR2ED and that the phosphatidyl inositol mannoside 2 (Pim2) is the minimal lipoarabinomannan structure for binding to hTLR2ED. Binding of hTLR2ED to Pim4, which contains a diacylglycer...

【 授权许可】

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