Transcriptional Changes of Blood Eosinophils after Methacholine Inhalation Challenge in Asthmatics:

Genomics Insights

Scott J.Tebbutt¹ 

关键词: asthma;    CCR3;    gene expression;    microarray;    peripheral blood;    protein ubiquitination;   

DOI  :  10.4137/GEI.S9125

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

BackgroundMethacholine challenge is commonly used within the asthma diagnostic algorithm. Methacholine challenge has recently been shown to induce airway remodelling in asthma via bronchoconstriction, without additional airway inflammation. We evaluated the effect of methacholine-induced bronchoconstriction on the peripheral whole-blood transcriptome.MethodsFourteen males with adult-onset, occupational asthma, 26–77 years of age, underwent methacholine inhalation challenges. The concentration of methacholine eliciting a ≥20% fall in FEV1 (PC20) was determined. Blood was collected immediately prior to and two hours after challenge. Complete blood counts and leukocyte differentials were obtained. Transcriptome analysis was performed using Affymetrix GeneChip® Human Gene 1.0 ST arrays. Data were analyzed using robust LEMMA and SAM. The cell-specific Significance Analysis of Microarrays (csSAM) algorithm was used to deconvolute the gene expression data according to cell type.ResultsMicroarray pathway analysis...

【 授权许可】

CC BY