Distinct carbohydrate and lipid-based molecular patterns within lipopolysaccharides from Burkholderia cepacia contribute to defense-associated differential gene expression in Arabidopsis thaliana:

Innate Immunity

Ntakadzeni EMadala¹ 

关键词: Arabidopsis thaliana;    Burkholderia cepacia;    innate;    immunity;    lipid A;    lipopolysaccharides;    MAMPs;    O-polysaccharide;    defense;    transcriptome;   

DOI  :  10.1177/1753425910392609

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Lipopolysaccharides are structural components within the cell walls of Gram-negative bacteria. The LPSs as microbe-associated molecular pattern (MAMP) molecules can trigger defense-related responses involved in MAMP-triggered immunity and basal resistance in plants, presumably from an initial perception event. LPS from Burkholderia cepacia as well as two fragments, the glycolipid, lipid A and the polysaccharide (OPS-core) chain, were used to treat Arabidopsis thaliana seedlings to evaluate the eliciting activities of the individual LPS sub-domains by means of Annealing Control Primer-based Differential Display transcript profiling. Genes found to be up-regulated encode for proteins involved in signal perception and transduction, transcriptional regulation and defense – and stress responses. Furthermore, genes encoding proteins involved in chaperoning, secretion, protein–protein interactions and protein degradation were differentially expressed. It is concluded that intact LPS, as well as the two sub-compo...

【 授权许可】

CC BY