Evaluation of biological efficacy of Trichoderma asperellum against tomato bacterial wilt caused by Ralstonia solanacearum

Egyptian Journal of Biological Pest Control

Srinivas Chowdappa¹  Soumya Krishnamurthy²  Narasimhamurthy Konappa³  Chandra Nayaka Siddaiah³  Niranjana Siddapura Ramachandrappa³ 

[1] Department of Microbiology and Biotechnology, Bangalore University, Bangalore, India;Department of Microbiology, Field Marshal K. M. Cariappa College, Constituent College of Mangalore University, Madikeri, India;Department of Studies in Biotechnology, University of Mysore, Mysore, India

关键词: Ralstonia solanacearum;    Induced systemic resistance (ISR);    Trichoderma asperellum;    Tomato yield;    Plant growth promoters;   

DOI  :  10.1186/s41938-018-0069-5

学科分类：动物科学

来源: Springer

【 摘 要 】

Bacterial wilt, caused by soilborne bacterium Ralstonia solanacearum, is one of the most severe diseases of tomato worldwide, and no successful control measures are available to date. In the present study, a sustainable alternative tool such as use of fungi from tomato rhizosphere is being utilized to combat the pathogen attack. The application of Trichoderma asperellum (T4 and T8) isolates delayed wilt development, effectively decreased the disease incidence, increased fruit yield, and improved plant growth promotion under field conditions. The T. asperellum treatment decreased the disease incidence by 51.06% (RS + T4) and 52.75% (RS + T8) in Bhoomishettihalli (BH) and 47.21% (RS + T4) and 46.83% (RS + T8) in Madanahalli (MH) plots, respectively when compared with the pathogen-treated plot in year 2014. Correspondent decreases in year 2015 were 50.69% (RS + T4) and 52.38% (RS + T8) in BH and 48.18% (RS + T4) and 49.22% (RS + T8) in MH plots. In year 2014, T. asperellum (T4 and T8) treatment enhanced the yield with 5.45 t/ha and 5.50 t/ha in BH plot and 6.66 t/ha and 6.93 t/ha in MH plot, respectively, when compared with infected plots. In year 2015, T. asperellum (T4 and T8) treatment enhanced the yield with 5.29 t/ha and 5.51 t/ha in BH plot and 5.82 t/ha and 5.66 t/ha in MH plot, respectively, when compared with infected plots. The disease control and yield enhancement were highest at T8, followed by T4. Increase in the level of peroxidase (POX), phenylalanine ammonium lyase (PAL), polyphenol oxidase (PPO), β-1,3-glucanase and total phenol activities at 12th, 10th, 14th, 12th, and 10th days, respectively, after pathogen inoculation was observed. This indicates the induction of plant resistance mechanism by T. asperellum against R. solanacearum in tomato plants under field conditions.

【 授权许可】

CC BY