Purification, physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves

卷:136

Xie, Jian-Hua ; Liu, Xin ; Shen, Ming-Yue ; Nie, Shao-Ping ; Zhang, Hui ; Li, Chang ; Gong, De-Ming ; Xie, Ming-Yong

Nanchang Univ

关键词: Polysaccharide;    Cyclocarya paliurus;    Purification;    Physicochemical property;    Characterisation;    Anticancer activity;   

DOI  :  10.1016/j.foodchem.2012.09.078

学科分类：食品科学和技术

【 摘 要 】

A Cyclocarya paliurus (Batal.) Iljinskaja polysaccharide (CPP) was isolated and purified by hot water extraction, ethanol precipitation, deproteinisation and anion-exchange chromatography. Its physicochemical properties were characterised by gel permeation chromatography (GPC), gas chromatography-mass spectrometry (GC-MS), thermal gravimetric analysis (TGA), Fourier transform infrared spectrometry (FTIR), UV-visible spectrophotometry, dynamic light scattering (DLS) and viscometry analysis. The anticancer effect of CPP in human gastric cancer HeLa cells was also evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MU) assay. The results showed that the molecular weight of CPP was 900 kDa, and it contained 64.8% total sugar, 23.5% uronic acid, 9.26% protein, and six kinds of monosaccharides, including glucose, rhamnose, arabinose, xylose, mannose and galactose, with molar percentages of 32.7%, 9.33%, 30.6%, 3.48%, 10.4%, and 13.5%, respectively. Furthermore, the results showed that CPP exhibited a strong inhibition effect on the growth of human gastric cancer HeLa cells. (C) 2012 Elsevier Ltd. All rights reserved.