Encapsulation of indole-3-carbinol and 3,3 '-diindolylmethane in zein/carboxymethyl chitosan nanoparticles with controlled release property and improved stability

卷:139

Luo, Yangchao ; Wang, Thomas T. Y. ; Teng, Zi ; Chen, Pei ; Sun, Jianghao ; Wang, Qin

Univ Maryland

关键词: Indole-3-carbinol;    Diindolylmethane;    Zein;    Carboxymethyl chitosan;    Encapsulation;    Stability;   

DOI  :  10.1016/j.foodchem.2013.01.113

学科分类：食品科学和技术

【 摘 要 】

Indole-3-carbinol (I3C) and 3,3'-diindolylmethane (DIM) are two bioactive compounds from Cruciferous vegetables. The stability of these compounds is a major challenge for their pharmaceutical applications. In this study, zein and zein/carboxymethyl chitosan (CMCS) nanoparticles were prepared to encapsulate I3C and DIM by a combined liquid-liquid phase separation and ionic gelation method. After zein nanoparticles were coated with CMCS, the zeta potential was decreased from around -10 to -20 mV, and encapsulation efficiency was greatly improved. Both nanoparticle formulations provided controlled release of I3C and DIM in PBS medium. Zein and zein/CMCS nanoparticles demonstrated similar protection for both I3C and DIM against ultraviolet (UV) light, attributed mainly to the contribution of the zein protein. Compared with zein nanoparticles, zein/CMCS nanoparticles exhibited better protection of I3C against degradation and better inhibition against its oligomerization to DIM under thermal condition (37 degrees C). Based on our results, the encapsulation of hydrophobic bioactives in zein/CMCS nanoparticles is a promising approach to improve their stability against harsh conditions and provide controlled release for food/pharmaceutical applications. (C) 2013 Elsevier Ltd. All rights reserved.