Monoclonal antibody-quantum dots CdTe conjugate-based fluoroimmunoassay for the determination of aflatoxin B-1 in peanuts

卷:146

Zhang, Zhaowei ; Li, Yuanyuan ; Li, Peiwu ; Zhang, Qi ; Zhang, Wen ; Hu, Xiaofeng ; Ding, Xiaoxia

Chinese Acad Agr Sci

关键词: Quantum dots CdTe;    Fluoroimmunoassay;    Aflatoxin B-1;    Peanuts;   

DOI  :  10.1016/j.foodchem.2013.09.048

学科分类：食品科学和技术

【 摘 要 】

A fluoroimmunoassay towards aflatoxin B-1 (AFB(1)) was presented using quantum dots as the fluorescent label. The CdTe QDs were successfully linked to the monoclonal antibody against AFB(1). Based on the conjugated complexes, a novel direct competitive fluorescence-linked immunosorbent assay (cFLISA) was developed for AFB(1) detection. The 50% inhibition value (IC50) of the cFLISA was 0.149 ng/mL in peanuts matrix. The method performance included the limit of detection (LOD) of 0.016 ng/mL and considerable recoveries of 85-117% at three fortification levels (0.075, 0.15, and 0.3 ng/g) from spiked AFB(1) blank peanuts samples, along with coefficients of variation (CVs) below 10%. The cFLISA provided an alternative of rapid and sensitive detection for AFB(1) and, moreover provided great potential for multiplexed mycotoxins determination simultaneously. (C) 2013 Elsevier Ltd. All rights reserved.