Composition of Lycium barbarum polysaccharides and their apoptosis-inducing effect on human hepatoma SMMC-7721 cells

卷:59

Zhang, Qian ; Lv, Xiaoling ; Wu, Tao ; Ma, Qian ; Teng, Anguo ; Zhang, Ying ; Zhang, Min

Tianjin Univ Sci & Technol

关键词: Lycium barbarum polysaccharide;    structure;    apoptosis;    cell cycle;    [Ca2+](i);    hepatoma SMMC-7721 cells;   

DOI  :  10.3402/fnr.v59.28696

学科分类：食品科学和技术

【 摘 要 】

Background: Lycium barbarum polysaccharide (LBP) is a natural functional component that has a variety of biological activities. The molecular structures and apoptosis-inducing activities on human hepatoma SMMC-7721 cells of two LBP fractions, LBP-d and LBP-e, were investigated. Results: The results showed that LBP-d and LBP-e both consist of protein, uronic acid, and neutral sugars in different proportions. The structure of LBP was characterized by gas chromatography, periodate oxidation, and Smith degradation. LBP-dwas composed of eight kinds of monosaccharides (fucose, ribose, rhamnose, arabinose, xylose, mannose, galactose, and glucose), while LBP-e was composed of six kinds of monosaccharides (fucose, rhamnose, arabinose, mannose, galactose, and glucose). LBP-d and LBP-e blocked SMMC-7721 cells at the G0/G1 and S phases with an inhibition ratio of 26.70 and 45.13%, respectively, and enhanced the concentration of Ca2+ in the cytoplasm of SMMC-7721. Conclusion: The contents of protein, uronic acid, and galactose in LBP-e were much higher than those in LBP-d, which might responsible for their different bioactivities. The results showed that LBP can be provided as a potential chemotherapeutic agent drug to treat cancer.