Binding of (-)-epigallocatechin-3-gallate with thermally-induced bovine serum albumin/iota-carrageenan particles

卷:168

Li, Jinbing ; Wang, Xiaoyong

E China Univ Sci & Technol

关键词: Biopolymer particles;    EGCG;    Fluorescence;    Binding constant;    Stability;   

DOI  :  10.1016/j.foodchem.2014.07.097

学科分类：食品科学和技术

【 摘 要 】

Novel thermally-induced BSA/iota-carrageenan particles are used as a protective carrier for (-)-epigallocatechin-3-gallate (EGCG). The addition of EGCG to BSA/iota-carrageenan particles can highly quench the intrinsic fluorescence of BSA, which is explained in terms of the binding of EGCG to the hydrophobic pockets of BSA mainly through the hydrophobic force. According to the double logarithm equation, the binding constant is determined as 1.1 x 10(8) M-1 for the binding of EGCG with BSA/iota-carrageenan particles. The high binding affinity is ascribed to both the molecular structure of EGCG and the partial unfolding state of BSA in BSA/iota-carrageenan particles. The circular dichroism spectra and calculated a-helix of BSA suggest that the bound EGCG leads to a more random secondary structure of BSA. Furthermore, BSA/iota-carrageenan particles are found to be superior to native BSA and pure BSA particles for improving the stability and radical scavenging activity of EGCG. (C) 2014 Elsevier Ltd. All rights reserved.