Variation of glucosinolates and quinone reductase activity among different varieties of Chinese kale and improvement of glucoraphanin by metabolic engineering

卷:168

Qian, Hongmei ; Sun, Bo ; Miao, Huiying ; Cai, Congxi ; Xu, Chaojiong ; Wang, Qiaomei

Zhejiang Univ

关键词: Chinese kale;    Glucosinolates;    Glucoraphanin;    Quinone reductase activity;    AOP2;    Metabolic engineering;   

DOI  :  10.1016/j.foodchem.2014.07.073

学科分类：食品科学和技术

【 摘 要 】

The variation of glucosinolates and quinone reductase (QR) activity in fourteen varieties of Chinese kale (Brassica oleracea var. alboglabra Bailey) was investigated in the present study. Results showed that gluconapin (GNA), instead of glucoraphanin (GRA), was the most predominant glucosinolate in all varieties, and QR activity was remarkably positively correlated with the glucoraphanin level. AOP2, a tandem 2-oxoglutarate-dependent dioxygenase, catalyzes the conversion of glucoraphanin to gluconapin in glucosinolate biosynthesis. Here, antisense AOP2 was transformed into Gailan-04, the variety with the highest gluconapin content and ratio of GNA/GRA. The glucoraphanin content and corresponding QR activity were notably increased in transgenic plants, while no significant difference at the level of other main nutritional compounds (total phenolics, vitamin C, carotenoids and chlorophyll) was observed between the transgenic lines and the wide-type plants. Taken together, metabolic engineering is a good practice for improvement of glucoraphanin in Chinese kale. (C) 2014 Elsevier Ltd. All rights reserved.