Differences in plasma metabolomics between sows fed DL-methionine and its hydroxy analogue reveal a strong association of milk composition and neonatal growth with maternal methionine nutrition

卷:113

Zhang, Xiaoling ; Li, Hao ; Liu, Guangmang ; Wan, Haifeng ; Mercier, Yves ; Wu, Caimei ; Wu, Xiuqun ; Che, Lianqiang ; Lin, Yan ; Xu, Shengyu ; Tian, Gang ; Chen, Daiwen ; Wu, De ; Fang, Zhengfeng

关键词: Methionine hydroxy analogue;    Plasma metabolomics;    Milk synthesis;    Sows;    Piglets;   

DOI  :  10.1017/S0007114514004036

学科分类：食品科学和技术

【 摘 要 】

The aim of the present study was to determine whether increased consumption of methionine as DL-methionine (DLM) or its hydroxy analogue DL-2-hydroxy-4-methylthiobutanoic acid (HMTBA) could benefit milk synthesis and neonatal growth. For this purpose, eighteen cross-bred (Landrace x Yorkshire) primiparous sows were fed a control (CON), DLM or HMTBA diet (n 6 per diet) from 0 to 14 d post-partum. At postnatal day 14, piglets in the HMTBA group had higher body weight (P=0.02) than those in the CON group, tended (P=0.07) to be higher than those in the DLM group, and had higher (P<0.05) mRNA abundance of jejunal fatty acid-binding protein 2, intestinal than those in the CON and DLM groups. Compared with the CON diet-fed sows, milk protein, non-fat solid, and lysine, histidine and ornithine concentrations decreased in the DLM diet-fed sows (P<0.05), and milk fat, lactose, and cysteine and taurine concentrations increased in the HMTBA diet-fed sows (P<0.05). Plasma homocysteine and urea N concentrations that averaged across time were increased (P<0.05) in sows fed the DLM diet compared with those fed the CON diet. Metabolomic results based on H-1 NMR spectroscopy revealed that consumption of the HMTBA and DLM diets increased (P<0.05) both sow plasma methionine and valine levels; however, consumption of the DLM diet led to lower (P<0.05) plasma levels of lysine, tyrosine, glucose and acetate and higher (P<0.05) plasma levels of citrate, lactate, formate, glycerol, myo-inositol and N-acetyl glycoprotein in sows. Collectively, neonatal growth and milk synthesis were regulated by dietary methionine levels and sources, which resulted in marked alterations in amino acid, lipid and glycogen metabolism.