Enantioselective chromatography in analysis of triacylglycerols common in edible fats and oils

卷:172

Kalpio, Marika ; Nylund, Matts ; Linderborg, Kaisa M. ; Yang, Baoru ; Kristinsson, Bjorn ; Haraldsson, Gudmundur G. ; Kallio, Heikki

关键词: Chiral;    Enantiomer;    High-performance liquid chromatography;    Stereoisomer;    Triacylglycerol;   

DOI  :  10.1016/j.foodchem.2014.09.135

学科分类：食品科学和技术

【 摘 要 】

Enantiomers of racemic triacylglycerol (TAG) mixtures were separated using two chiral HPLC columns with a sample recycling system and a UV detector. A closed system without sample derivatisation enabled separation and identification by using enantiopure reference compounds of eleven racemic TAGs with C12-C22 fatty acids with 0-2 double bonds. The prolonged separation time was compensated for by fewer pretreatment steps. Presence of one saturated and one unsaturated fatty acid in the asymmetric TAG favoured the separation. Enantiomeric resolution, at the same time with stronger retention of TAGs, increased with increasing fatty acid chain length in the sn-1(3) position. Triunsaturated TAGs containing oleic, linoleic or palmitoleic acids did not separate. The elution order of enantiomers was determined by chemoenzymatically synthesised enantiopure TAGs with a co-injection method. The method is applicable to many natural fats and oils of low unsaturation level assisting advanced investigation of lipid synthesis and metabolism. (C) 2014 Elsevier Ltd. All rights reserved.