Bioactive amines in sorghum: Method optimisation and influence of line, tannin and hydric stress

卷:173

Paiva, Caroline Liboreiro ; Evangelista, Warlley Pinheiro ; Vieira Queiroz, Valeria Aparecida ; Abreu Gloria, Maria Beatriz

关键词: Bioactive amines;    Polyamines;    Sorghum bicolor L.;    Extraction;    Hydric stress;   

DOI  :  10.1016/j.foodchem.2014.10.039

学科分类：食品科学和技术

【 摘 要 】

The profile and levels of bioactive amines in different sorghum lines were reported for the first time. The amines were quantified by ion-pair HPLC, post-column derivatisation with o-phthalaidehyde and fluorimetric detection. The extraction procedure was optimised: 420 gm particle size, extraction with 5% trichloroacetic acid and three extractions. The screening of 22 sorghum lines showed that four of the ten amines investigated were detected. Spermine and spermidine were the prevalent amines (100%), followed by putrescine (77%) and cadaverine (14%). Total amines ranged from 5.8 to 41.4 mg/100 g, and the polyamines represented 60-100% of the total. Sorghum without tannin had higher amines levels compared to sorghum with tannin and cadaverine was specific to samples without tannin. Hydric stress caused accumulation of spermidine in the grains and affected the levels of other amines at rates depending on the presence or not of tannin. Sorghum is a significant source of polyamines. (C) 2014 Elsevier Ltd. All rights reserved.