Purification and characterisation of arabinoxylan arabinofuranohydrolase I responsible for the filterability of barley malt

卷:174

Li, Xiaomin ; Gao, Fei ; Cai, Guolin ; Jin, Zhao ; Lu, Jian ; Dong, Jianjun ; Yin, Hua ; Yu, Junhong ; Yang, Mei

关键词: Barley malt;    Filterability;    Dan'er;    Arabinoxylan arabinofuranohydrolase;    Arabinoxylan;   

DOI  :  10.1016/j.foodchem.2014.11.024

学科分类：食品科学和技术

【 摘 要 】

Dan'er is a widely grown malt barley cultivar in China, but its filterability defects have severely impeded its application in beer brewing. Previous investigations have suggested that we should identify the malt filterability correlated proteins, one of which was postulated to be arabinoxylan arabinofuranohydrolase I (AXAH-I). To verify this hypothesis, we purified AXAH-I from Dan'er malt, characterised its enzyme performance, and investigated its influence on filterability by adding different amounts of purified enzyme to the mash. With 6 mU g(-1) malt AXAH-I supplemented, the wort separation rate increased by 31.8%, viscosity decreased by 3.6%, and the endosperm reserve contents declined concomitantly. Unexpectedly, the wort turbidity increased with increasing AXAH-I. We also tried to optimise the use of currently available commercial enzyme products for filterability improvement in beer brewing, by supplementing them with purified AXAH-I and beta-amylase. AXAH-I could be a functional component for novel commercial enzyme products in the beer industry. (C) 2014 Elsevier Ltd. All rights reserved.